Kept out of the side by Rob Andrew, Barnes had been due for his 28th spell on the substitutes' bench, but the injury, sustained Monday, will narrow England's replacement options.
Prop Nick Popplewell, who played for the British Lions against New Zealand last year, has ruled himself out of Ireland's tour to Australia in May and June.
"I've been playing more or less continuous rugby for three years without a break," Popplewell said Monday. "This season I have had continual hamstring trouble and I just feel that I need a rest from the game."
A Message from The Moscow Times:
Dear readers,
We are facing unprecedented challenges. Russia's Prosecutor General's Office has designated The Moscow Times as an "undesirable" organization, criminalizing our work and putting our staff at risk of prosecution. This follows our earlier unjust labeling as a "foreign agent."
These actions are direct attempts to silence independent journalism in Russia. The authorities claim our work "discredits the decisions of the Russian leadership." We see things differently: we strive to provide accurate, unbiased reporting on Russia.
We, the journalists of The Moscow Times, refuse to be silenced. But to continue our work, we need your help.
Your support, no matter how small, makes a world of difference. If you can, please support us monthly starting from just $2. It's quick to set up, and every contribution makes a significant impact.
By supporting The Moscow Times, you're defending open, independent journalism in the face of repression. Thank you for standing with us.
Remind me later.