The government’s Covid-19 information center said Moscow had recorded 3,445 new cases in the past 24 hours.
Russia has the fifth-highest number of recorded Covid-19 cases in the world and the highest death toll in Europe.
Russian officials have raised alarm in recent days over rising case numbers.
Anna Popova, the head of the Rospotrebnadzor health watchdog which is leading the country’s response to the virus, said the growth in new infections was “intense” in 36 of Russia’s 85 regions.
She said the spike could be attributed to seasonal respiratory infections and warned Russian regional hospitals to be ready for an influx of patients.
Last week, dozens of people in President Vladimir Putin’s closest circle were diagnosed with Covid-19, prompting the president to self-isolate.