A draw against CSKA Moscow on Wednesday will be enough for Wolfsburg to secure second place in Group B and a spot in the knockout round of the Champions League.
CSKA, meanwhile, must beat Wolfsburg to keep alive its chances of qualifying.
Manchester United has already advanced to the last 16, topping Group B with 10 points from four games.
Wolfsburg is second with seven points, three ahead of CSKA, and avoiding defeat at Luzhniki Stadium on Wednesday will guarantee the German champion the group's second spot in the knockout stage.
Wolfsburg midfielder Christian Gentner said the visitors won't be holding out for a draw.
"Playing for a draw has never worked out well," he said. "We want to win there."
Wolfsburg beat CSKA 3-1 in the group stage opener in September.
CSKA will be playing under new coach Leonid Slutsky, who took over from fired Spaniard Juande Ramos last month.
"We understand what result we need," Slutsky said. "We need only victory and that's what we're aiming for."
CSKA, which edged crosstown rival Spartak 3-2 on Saturday to stay in contention for third place in the Russian league with one game left in the season, reported no injuries, but will be without suspended Lithuanian international defender Deividas Semberas.
"It won't be easy to substitute Semberas," Slutsky said. "In the games under my helm he played well."
Wolfsburg is aiming to bounce back from a 3-2 weekend league defeat at home to Nuremberg and comes to Moscow without suspended Brazilian striker Grafite, who was sent off against Besiktas last month.
"We absolutely have to do better on Wednesday," Grafite said. "I am very sad that I can't play, but I am sure the team will play a good game without me."
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